expression profiling of genes involved in signaling process in aegilops tauschii under salinity stress

نویسندگان

مهدی منصوری

دانشجوی دکتری، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج محمد رضا نقوی

استاد پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج هوشنگ علیزاده

استادیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج قاسم محمدی نژاد

دانشیار گروه زراعت و اصلاح نباتات دانشگاه شهید باهنر کرمان سید احمد موسوی

چکیده

salinization of crop lands is one of the most important reasons for reduction in productivity and threats food security. activation of signaling process in plants is the first step during sense environmental cues and stress signals. identification of the components of plant intracellular signaling mechanisms, pathways, and networks in response to salt stress in aegilops tauschii (the d genome donor of bread wheat) by transcriptome analysis tools such as rna seq method is very worthwhile. rna seq method and functional annotation of data by mapman suite is an efficient strategy to functional genomics analyses. in order to identify and evaluate some genes involved in signaling process, we performed library construction for two cdna libraries from leaves of seedlings treated with 200 mm nacl and control. results of differential expression showed that out of 4506 differentially expressed genes, 603 (11/59%) bins assigned to signaling process. it also became clear that receptor kinases are the largest number (222 transcripts) of differentially expressed genes in signaling process components. this study is a fundamental research that provide worthwhile functional sequences for gene transferring study to improve salinity tolerant in bread wheat.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Investigation in expression of key genes involved in sanguinarine biosynthetic pathway in Papaver genus under drought and salinity

The presence of morphine alkaloids, anti-microbial and anti-cancer metabolite, sanguinarine, in different species of Papaver genus has made it one of the most valuable plants in the pharmaceutical industry.With regard to the influence of drought and salinity on the increase of secondary metabolites, the present study attempted to investigate the effect of the mentioned stresses on the key genes...

متن کامل

Key Genes Involved in Wheat Response to Salinity Stress and Mapping their Gene Network

Extended Abstract Introduction and Objective: Considering the importance of salinity in wheat and the multigene nature of this trait, the present study was conducted to investigate the expression of key genes involved in the response of wheat to this stress and to create their network. Material and Methods: In this study, the expression of key genes (HKT, DREB, bZIP, NAC, and WARKY) involved...

متن کامل

Expression of related proteins and aquaporin genes in grape (Vitis vinifera L.) under salinity sress

Due to worldwide increasing of salinity, the identification of genes conferring tolerance to plants is important. The aim of this study was to investigate salinity effects on the expression of three genes-related to proteins and aquaporin in grape (Vitis vinifera L.). Based on screening study on 18 grape genotypes, H6 and Gharashani that showed lower decrease in water potential, leaf area, leaf...

متن کامل

Global gene expression profiling related to temperature-sensitive growth abnormalities in interspecific crosses between tetraploid wheat and Aegilops tauschii

Triploid wheat hybrids between tetraploid wheat and Aegilops tauschii sometimes show abnormal growth phenotypes, and the growth abnormalities inhibit generation of wheat synthetic hexaploids. In type II necrosis, one of the growth abnormalities, necrotic cell death accompanied by marked growth repression occurs only under low temperature conditions. At normal temperature, the type II necrosis l...

متن کامل

Characterization of novel LMW-GS genes at Glu-D3 locus on chromosome 1D in Aegilops tauschii.

The objectives of this study were to clarify the relationship between LMW-GS Glu-D3 gene of Ae. tauschii registered in GenBank and the six Glu-D3 genes including 12 allelic variants of common wheat characterized in our previous studies, and identify novel Glu-D3 genes and haplotypes from Ae. tauschii using gene specific PCR amplification. By searching the NCBI database, 13 LMW-GS genes/pseudoge...

متن کامل

The effect of probiotics on signaling pathways of genes involved in depression: review article

Depression is the psychological disorder which caused by chronic stress and extensive communication network between the gastrointestinal system via the “gut–brain-microbiota axis”. Depression can systematically activate/deactivate many genes and signaling molecules involved in the pathogenesis of the gastrointestinal disease. Whereas, most of the anti-depressant drugs suppress the depression sy...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
علوم گیاهان زراعی ایران

جلد ۴۷، شماره ۲، صفحات ۲۰۵-۲۱۶

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023